20-P017 Visualizing mesodermal cell migration in chicken embryo

نویسندگان

  • Yukiko Nakaya
  • Erike W. Sukowati
  • Guojun Sheng
چکیده

The WAS protein (WASp) coordinates actin polymerization at the leading edge of migrating cells and is defective in patients suffering from Wiskott Aldrich syndrome, resulting in severe immunodeficiency. To model this disease we are using translucent Zebrafish larvae and have begun to characterise a newly derived WASp mutant. The mutant codes for a heavily truncated protein, which we are assaying for functional activity. Using live Confocal imaging techniques we have analysed the wound inflammatory response, in wild type and mutant larvae, as well as the capability for mutant leukocytes to phagocytose and kill microbes, and the blood clotting capacity of these fish. We am currently attempting a ‘‘rescue’’ of the mutant phenotype with a range of human WASp genes reflecting the spectrum of the human disease.

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عنوان ژورنال:
  • Mechanisms of Development

دوره 126  شماره 

صفحات  -

تاریخ انتشار 2009