20-P017 Visualizing mesodermal cell migration in chicken embryo
نویسندگان
چکیده
The WAS protein (WASp) coordinates actin polymerization at the leading edge of migrating cells and is defective in patients suffering from Wiskott Aldrich syndrome, resulting in severe immunodeficiency. To model this disease we are using translucent Zebrafish larvae and have begun to characterise a newly derived WASp mutant. The mutant codes for a heavily truncated protein, which we are assaying for functional activity. Using live Confocal imaging techniques we have analysed the wound inflammatory response, in wild type and mutant larvae, as well as the capability for mutant leukocytes to phagocytose and kill microbes, and the blood clotting capacity of these fish. We am currently attempting a ‘‘rescue’’ of the mutant phenotype with a range of human WASp genes reflecting the spectrum of the human disease.
منابع مشابه
20-P019 Study of pallio-subpallial neural tangential migration
tem to observe mesoderm cell migration in chicken embryo using live imaging. In early-stage chicken embryo, mesoderm cells move away from the primitive streak and follow stereotyped migratory route according to their dorsal-ventral fate. Immunohistochemical studies and live cell imaging with fluorescent labeled nucleus and Golgi complex showed a consistent positioning of Golgi complex at the re...
متن کامل20-P018 Live imaging the cortical lamination in slice culture of embryonic mouse cerebrum
tem to observe mesoderm cell migration in chicken embryo using live imaging. In early-stage chicken embryo, mesoderm cells move away from the primitive streak and follow stereotyped migratory route according to their dorsal-ventral fate. Immunohistochemical studies and live cell imaging with fluorescent labeled nucleus and Golgi complex showed a consistent positioning of Golgi complex at the re...
متن کاملTransplantation studies to investigate mesoderm-ectoderm adhesive cell interactions during gastrulation.
Experiments involving transplantation of the roof of the blastocoel in Rana pipiens embryos reveal that the inner surface of the roof of the blastocoel must be coated with a fibrous extracellular matrix (F-ECM) to serve as a substratum for mesodermal cell migration. When the roof of the blastocoel is inverted the original outer surface, now projecting toward the blastocoel, does not become coat...
متن کامل20-P020 An involvement of cue-induced regulation of NFPC in retinal growth cone navigation
tem to observe mesoderm cell migration in chicken embryo using live imaging. In early-stage chicken embryo, mesoderm cells move away from the primitive streak and follow stereotyped migratory route according to their dorsal-ventral fate. Immunohistochemical studies and live cell imaging with fluorescent labeled nucleus and Golgi complex showed a consistent positioning of Golgi complex at the re...
متن کاملAn ECM substratum allows mouse mesodermal cells isolated from the primitive streak to exhibit motility similar to that inside the embryo and reveals a deficiency in the T/T mutant cells.
The mesodermal cell layer is created by ingression and migration of the cells from the primitive streak region in mouse embryos on day 7 of pregnancy. In order to study the mechanisms of mesodermal cell migration during development, the mesodermal cells isolated from the primitive streak were cultured on various substrata, and cell behaviour and motility were analysed with a time-lapse video sy...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Mechanisms of Development
دوره 126 شماره
صفحات -
تاریخ انتشار 2009